High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production

Xiaojie Duan; Yu Liu; Xin You; Zhengqiang Jiang; Shaoxiang Yang; Shaoqing Yang

doi:10.1186/s13068-017-0912-z

High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production

Biotechnol Biofuels. 2017 Sep 19:10:223. doi: 10.1186/s13068-017-0912-z. eCollection 2017.

Authors

Xiaojie Duan^{1

2}, Yu Liu^{1

2}, Xin You^{1

3}, Zhengqiang Jiang^{1

2}, Shaoxiang Yang⁴, Shaoqing Yang^{1

2}

Affiliations

¹ Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100083 China.
² College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083 China.
³ College of Engineering, China Agricultural University, Beijing, 100083 China.
⁴ Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University (BTBU), Beijing, 100048 China.

Abstract

Background: Butyl butyrate has been considered as a promising fuel source because it is a kind of natural ester which can be converted from renewable and sustainable lignocellulosic biomass. Compared with the conventional chemical methods for butyl butyrate production, the enzymatic approach has been demonstrated to be more attractive, mainly owing to the mild reaction conditions, high specificity, low energy consumption, and environmental friendliness. Cutinases play an important role in the butyl butyrate production process. However, the production level of cutinases is still relatively low. Thus, to identify novel cutinases suitable for butyl butyrate synthesis and enhance their yields is of great value in biofuel industry.

Results: A novel cutinase gene (McCut) was cloned from a thermophilic fungus Malbranchea cinnamomea and expressed in Pichia pastoris. The highest cutinase activity of 12, 536 U/mL was achieved in 5-L fermentor, which is by far the highest production for a cutinase. McCut was optimally active at pH 8.0 and 45 °C. It exhibited excellent stability within the pH range of 3.0-10.5 and up to 75 °C. The cutinase displayed broad substrate specificity with the highest activity towards p-nitrophenyl butyrate and tributyrin. It was capable of hydrolyzing cutin, polycaprolactone, and poly(butylene succinate). Moreover, McCut efficiently synthesized butyl butyrate with a maximum esterification efficiency of 96.9% at 4 h. The overall structure of McCut was resolved as a typical α/β-hydrolase fold. The structural differences between McCut and Aspergillus oryzae cutinase in groove and loop provide valuable information for redesign of McCut. These excellent features make it useful in biosynthesis and biodegradation fields.

Conclusions: A novel cutinase from M. cinnamomea was identified and characterized for the first time. High-level expression by P. pastoris is by far the highest for a cutinase. The enzyme exhibited excellent stability and high esterification efficiency for butyl butyrate production, which may make it a good candidate in biofuel and chemical industries.

Keywords: Butyl butyrate; Crystal structure; Cutinase; High-level expression; Malbranchea cinnamomea; Stability.